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suspension adapted hek293 ebna cells  (ATCC)


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    Structured Review

    ATCC suspension adapted hek293 ebna cells
    Suspension Adapted Hek293 Ebna Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/suspension+adapted+hek293+ebna+cells/293+c18/us12006367-371-13-16
    Average 95 stars, based on 201 article reviews
    suspension adapted hek293 ebna cells - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC®-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Article Title: Development of ISB 1442, a CD38 and CD47 bispecific biparatopic antibody innate cell modulator for the treatment of multiple myeloma
    Article Snippet: .. Daratumumab was purchased from Janssen Biotech Inc. Anti-CD47 monoclonal antibody (hu5F9) was produced in house by transient expression into suspension-adapted HEK293-EBNA cells (catalog no. ATCC-CRL-10852, LGC Standards, Teddington, UK) using polyethyleneimine. ..

    Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Suspension:

    Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC®-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Article Title: Development of ISB 1442, a CD38 and CD47 bispecific biparatopic antibody innate cell modulator for the treatment of multiple myeloma
    Article Snippet: .. Daratumumab was purchased from Janssen Biotech Inc. Anti-CD47 monoclonal antibody (hu5F9) was produced in house by transient expression into suspension-adapted HEK293-EBNA cells (catalog no. ATCC-CRL-10852, LGC Standards, Teddington, UK) using polyethyleneimine. ..

    Article Title: A single mutation increases heavy-chain heterodimer assembly of bispecific antibodies by inducing structural disorder in one homodimer species
    Article Snippet: PCR products were cloned into a modified pcDNA3.1 plasmid (Invitrogen) carrying oriP which is the origin of plasmid replication of Epstein–Barr virus. .. Heterodimeric BEAT antibodies were recombinantly expressed by co-transfecting Hcs based on BEAT (A) and BEAT (B), as well as the cognate Lc into suspension-adapted HEK293-EBNA cells (catalog. no. ATCC-CRL-10852, LGC Standards, Teddington, UK) using polyethyleneimine. ..

    Article Title: A single mutation increases heavy-chain heterodimer assembly of bispecific antibodies by inducing structural disorder in one homodimer species
    Article Snippet: PCR products were cloned into a modified pcDNA3.1 plasmid (Invitrogen AG, Basel, Switzerland) carrying oriP which is the origin of plasmid replication of Epstein-Barr virus. .. Heterodimeric BEAT antibodies were recombinantly expressed by co-transfecting Hcs based on BEAT (A) and BEAT (B) as well as the cognate Lc into suspension-adapted HEK293-EBNA cells (cat. no.: ATCC-CRL10852, LGC Standards, Teddington, UK) using polyethyleneimine. ..

    Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
    Article Snippet: .. For transient expression, equal quantities of each engineered chains vectors were co-transfected into suspension-adapted HEK293-EBNA cells (ATCC-LGL standards, Teddington, UK; Cat. No: CRL-10852) using Polyethyleneimine (PEI; Sigma, Buchs, Switzerland). ..

    Polymerase Chain Reaction:

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Amplification:

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Construct:

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Plasmid Preparation:

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Produced:

    Article Title: A bispecific IgG format containing four independent antigen binding sites
    Article Snippet: Stable cell lines were developed by addition of selection pressure, 600 μg/mL Hygromycin (Thermo Fisher) and limiting dilution of surviving transfected cells. .. Genes encoding the extracellular domain (ECD), PCR amplified from full length constructs above, of CD40 (aa 1-193), OX40 (aa1-216) or 4-1BB (aa1-186), respectively, were ligated into an expression vector (in-house developed) containing a C-terminal 8x His-tag and produced in suspension-adapted HEK293 EBNA cells (ATCC, cat no. ATCC-CRL-10852). .. The proteins were purified from the culture medium (FreeStyle293 expression Medium, Thermo Fisher Scientific) with Ni-NTA Affinity Chromatography (ÄKTA purifier, GE Healthcare).

    Article Title: Development of ISB 1442, a CD38 and CD47 bispecific biparatopic antibody innate cell modulator for the treatment of multiple myeloma
    Article Snippet: .. Daratumumab was purchased from Janssen Biotech Inc. Anti-CD47 monoclonal antibody (hu5F9) was produced in house by transient expression into suspension-adapted HEK293-EBNA cells (catalog no. ATCC-CRL-10852, LGC Standards, Teddington, UK) using polyethyleneimine. ..



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